Search results for "active transport"
showing 10 items of 80 documents
Survivin’s Dual Role: An Export’s View
2007
Survivin is proposed to function as a mitotic regulator and an apoptosis inhibitor during development and pathogenesis. As such, survivin has aroused keen interest in disparate areas of basic and translational research. Survivin acts as a subunit of the chromosomal passenger complex (CPC), composed of the mitotic kinase Aurora-B, Borealin and INCENP, and is essential for proper chromosome segregation and cytokinesis. Our recent findings indicate that the nuclear export receptor Crm1 is critically involved in tethering the CPC to the centromere by interacting with a leucine-rich nuclear export signal (NES), evolutionary conserved in all mammalian survivin proteins. In addition, the survivin/…
Environmental Conditions, Air Pollutants, and Airways
2019
Air pollution is a major problem worldwide, which could be even more serious for athletes who train in urban environments. Exercise increases minute ventilation and exposure to pollutants, but the literature on the effects of air pollution in athletes is relatively scarce, with the exception of chlorine exposure in athletes of aquatic sports and air pollution secondary to ice resurfacing in athletes performing in ice arenas. Although air pollution may exert detrimental effects on athletic performance, little has been published on this topic. The largest body of information regards the impact of air pollution during urban active transport, i.e., walking and cycling in cities, due to the pote…
Cytoplasmic Parvovirus Capsids Recruit Importin Beta for Nuclear Delivery
2019
Parvoviruses are an important platform for gene and cancer therapy. Their cell entry and the following steps, including nuclear import, are inefficient, limiting their use in therapeutic applications. Two models exist on parvoviral nuclear entry: the classical import of the viral capsid using nuclear transport receptors of the importin (karyopherin) family or the direct attachment of the capsid to the nuclear pore complex leading to the local disintegration of the nuclear envelope. Here, by laser scanning confocal microscopy and in situ proximity ligation analyses combined with coimmunoprecipitation, we show that infection requires importin β-mediated access to the nuclear pore complex and …
The mitotic kinase Aurora-A promotes distant metastases by inducing epithelial-to-mesenchymal transition in ERα+ breast cancer cells
2013
In this study, we demonstrate that constitutive activation of Raf-1 oncogenic signaling induces stabilization and accumulation of Aurora-A mitotic kinase that ultimately drives the transition from an epithelial to a highly invasive mesenchymal phenotype in estrogen receptor α-positive (ERα(+)) breast cancer cells. The transition from an epithelial- to a mesenchymal-like phenotype was characterized by reduced expression of ERα, HER-2/Neu overexpression and loss of CD24 surface receptor (CD24(-/low)). Importantly, expression of key epithelial-to-mesenchymal transition (EMT) markers and upregulation of the stemness gene SOX2 was linked to acquisition of stem cell-like properties such as the ab…
Test-retest reliability and construct validity of the ENERGY-child questionnaire on energy balance-related behaviours and their potential determinant…
2011
Published version of an article in the journal: International Journal of Behavioral Nutrition and Physical Activity. Also available from the publihser:http://dx.doi.org/10.1186/1479-5868-8-136 BACKGROUND:Insight in children's energy balance-related behaviours (EBRBs) and their determinants is important to inform obesity prevention research. Therefore, reliable and valid tools to measure these variables in large-scale population research are needed.OBJECTIVE:To examine the test-retest reliability and construct validity of the child questionnaire used in the ENERGY-project, measuring EBRBs and their potential determinants among 10-12 year old children.METHODS:We collected data among 10-12 yea…
Hypoxia-Induced miR-675-5p Supports β-Catenin Nuclear Localization by Regulating GSK3-β Activity in Colorectal Cancer Cell Lines
2020
The reduction of oxygen partial pressure in growing tumors triggers numerous survival strategies driven by the transcription factor complex HIF1 (Hypoxia Inducible Factor-1). Recent evidence revealed that HIF1 promotes rapid and effective phenotypic changes through the induction of non-coding RNAs, whose contribution has not yet been fully described. Here we investigated the role of the hypoxia-induced, long non-coding RNA H19 (lncH19) and its intragenic miRNA (miR-675-5p) into HIF1-Wnt crosstalk. During hypoxic stimulation, colorectal cancer cell lines up-regulated the levels of both the lncH19 and its intragenic miR-675-5p. Loss of expression experiments revealed that miR-675-5p inhibitio…
A GIS-based method for evaluating the walkability of a pedestrian environment and prioritised investments
2020
Abstract Despite the large investments made in the construction and modernisation of railway infrastructure, poor quality pedestrian routes may discourage users from using public transport. In fact, very little attention is generally paid to pedestrian mobility. Therefore, a method for evaluating the quality of pedestrian paths and the accessibility to railway stations has been developed. This method considers the main factors influencing the walkability of an urban area and makes it possible to establish the priorities for intervention, i.e. to identify the arcs of a pedestrian network that require prioritised action. The methodology is a decision support tool that can be used by policymak…
Changes in physical activity domains during the transition out of high school: Psychosocial and environmental correlates
2015
Background:This study examined changes in multiple physical activity domains during the transition out of high school and psychosocial and environmental determinants of these changes.Methods:A 1-year prospective study was designed. The baseline sample was composed of 244 last-year high school students (58.6% female) from Valencia, Spain. Follow-up rate was 46%. Physical activity and potential determinants were measured by the Global Physical Activity Questionnaire and other evaluated scales in 2 waves.Results:Total physical activity and active commuting (AC) decreased, respectively, by 21% and 36%, only in males. At time 1, access to car/motorbike (inverse), planning/psychosocial barriers (…
The importance of tight junctions in the blood-brain barrier
2021
There are two barriers in the central nervous system (CNS) responsible for maintaining the homeostatic balance of the human body’s internal environment in relation to external conditions: the blood-brain barrier (BBB) and the blood-cerebrospinal fluid barrier (BCSFB). The blood-brain barrier is a physical barrier with a fixed location between the blood and the nervous tissue. Its basic structural elements include endothelial cells of the brain capillaries, astrocytes and pericytes. The blood-brain barrier is the barrier between the blood and neurons which isolates the central nervous system from the rest of the body in a unique manner. The blood-brain barrier is formed by astrocytes and per…
Dissection of human papillomavirus type 33 L2 domains involved in nuclear domains (ND) 10 homing and reorganization
2003
Abstract We have recently shown that the minor capsid protein L2 of human papillomavirus type 33 (HPV33) recruits the transcriptional repressor Daxx into nuclear domains (ND) 10 and causes the loss of the transcriptional activator Sp100 from these subnuclear structures (Florin et al., 2002b) . In order to dissect L2 domains involved in nuclear translocation, ND10 homing, loss of Sp100, and recruitment of Daxx, a detailed deletion mutagenesis of L2 was performed. Using immunofluorescence and green fluorescent protein fusions, we have identified two nuclear localization signals (NLS) in the central and C-terminal part of L2, respectively, homologous to previously identified NLS in HPV6B L2 (S…